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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human recombinant protein fragment corresponding to amino acids 2-174 of human RBM8A (NP_005096) produced in E.coli.
- 形态:
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
- 保存条件:
Store at -20°C as received.
- 克隆性:
Monoclonal
- 适应物种:
Human, Rat, Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA812496AM
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
Y14
- 抗体英文名:
RBM8A mouse monoclonal antibody,clone OTI10B10, Biotinylated
- 抗体名:
RBM8A mouse monoclonal antibody,clone OTI10B10, Biotinylated
- 规格:
100 ul
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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