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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant fusion protein of SARS-CoV-2 Spike RBD protein and virion surface domain of membrane protein (M-protein) produced in HEK293
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 保存条件:
Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
- 克隆性:
Monoclonal
- 适应物种:
SARS-CoV-2
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
CF814511
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
ELISA
- 靶点:
SARS-CoV-2 Spike Protein
- 抗体英文名:
Carrier-free (BSA/glycerol-free) SARS-CoV-2 Spike protein mouse monoclonal antibody, clone OTI1H6
- 抗体名:
Carrier-free (BSA/glycerol-free) SARS-CoV-2 Spike protein mouse monoclonal antibody, clone OTI1H6
- 规格:
100 ug
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文献和实验Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Western Blotting with Monoclonal Ant
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. Polyacrylamide Gel
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