- ￥2756
- OriGene
- 美国
- R1387P
- 2026年04月01日
- Donkey
- Goat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Goat IgG whole molecule.
- 形态：
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.State: Aff - PurifiedState: Liquid (sterile filtered) Fab fragments.
- 保存条件：
Store the antibody (undiluted) at 2-8°C for one monthor (in aliquots) at -20°C for longer.Dilute only prior to immediate use.Avoid cycles of freezing and thawing.
- 克隆性：
Polyclonal
- 适应物种：
Goat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
R1387P
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Donkey
- 抗体英文名：
Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Aff - Purified
- 抗体名：
Goat IgG (H+L chain), Fab Fragment, adsorbed donkey polyclonal antibody, Aff - Purified
- 规格：
1 mg
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
2c + ++ Sources of information: Akerstrom, B., Brodin, T., Reis, K., and Bjorck, L. 1985. Protein G: A powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135:2589-2592. Akerstrom, B. and Bjorck, L. 1986
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemisty-Fluorescence Protocol
: If using R&D Systems biotinylated antibody skip steps 9 and 10 and continue. Incubate each cell spot on slides for 30 minutes at room temperature with 15 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted
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