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- 文献和实验
- 技术资料
- 免疫原:
Highly purified monoclonal IgA2 isolated from pooled Human serum
- 形态:
PBS, pH 7.2 without preservatives and foreign proteinsState: Azide FreeState: Lyophilized purified Ig fraction
- 保存条件:
Prior to reconstitution store at 2-8°C.Following reconstitution store undiluted at 2-8°C for one weekor (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM20247PU-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
ELISA, IF, IP, WB
- 抗体英文名:
Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Azide Free
- 抗体名:
Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Azide Free
- 规格:
200 ug
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文献和实验Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
antibody against surface marker of interest 0.1% paraformaldehyde in PBS 70% EtOH in glycerol (stored at 4°C) staining buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 m g/ml solution with 100 U/ml DNase-free
Immunodetection of cyclin D1 and D2/D3 using 488/630 nm dual laser flow cytometr(图)
D2/D3 (cat. no. 14711A, clone G107-22). Other cyclin antibodies may be appropriate as well. Cy5-conjugated anti-mouse IgG (Jackson ImmunoResearch and Caltag) 0.5% paraformaldehyde in PBS 80% EtOH in ddH20 (stored at -20℃) cyclin staining
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