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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mouse Monoclonal eRFP Antibody, Magnetic Beads
- 抗体英文名:
Mouse Monoclonal eRFP Antibody, Magnetic Beads
- 靶点:
eRFP
- 应用范围:
IP, Protein Purification
- 宿主:
Mouse
- 适应物种:
eRFP
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA183034
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
- 免疫原:
This antibody is raised against a recombinant protein of eRFP expressed in HEK293 cells.
- 规格:
1 ml
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文献和实验Mouse B cell isolation with magnetic beads
实验概要 Isolate B cells from total mouse cells Praparation: 1.Enough MACS; put RPMI into water bath. 2.Put some MACS in 15 ml tube or 1.5 ml tube from cell room
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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