- ￥5640
- OriGene
- AP31535HR-N
- 美国
- 2026年04月01日
- ELISA, ID, IF, IHC, WB
- Goat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Mouse Kappa Light Chain (free and bound) goat polyclonal antibody, HRP
- 抗体英文名：
Mouse Kappa Light Chain (free and bound) goat polyclonal antibody, HRP
- 应用范围：
ELISA, ID, IF, IHC, WB
- 宿主：
Goat
- 适应物种：
Mouse
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AP31535HR-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 克隆性：
Polyclonal
- 保存条件：
Store lyophilized at 2-8°C for 6 months or at -20°C long term.After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.Avoid repeated freezing and thawing.
- 形态：
PBS, pH 7.2No preservative added, as it may interfere with the antibody activity. No foreign proteins added.Label: HRPState: Lyophilised purified Hyperimmune Ig fractionStabilizer: NonePreservative: NoneLabel: Horseradish PeroxidaseMolar radio: 1.7
- 免疫原：
A pool of purified Bence Jones kappa proteins isolated from mouse urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 规格：
1 ml
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文献和实验Polyclonal and Monoclonal Antibodies
Polyclonal Antibody Immunization of Mice: Injections should be made at intervals of at least two weeks.. We have been using either of the two following adjuvants with success: Freund adjuvant and MPL+TDM adjuvant ( RIBI ImmunoChem
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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