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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Carrier-free (BSA/glycerol-free) EMG1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)
- 抗体英文名:
Carrier-free (BSA/glycerol-free) EMG1 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)
- 靶点:
EMG1
- 应用范围:
FC, IF, WB
- 宿主:
Mouse
- 适应物种:
Human, Dog, Monkey, Mouse, Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
CF501209
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原:
Full length human recombinant protein of human EMG1(NP_006322) produced in HEK293T cell.
- 规格:
100 ug
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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