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- 文献和实验
- 技术资料
- 抗体名:
TLE1 biotinylated mouse monoclonal detection antibody, validated for ELISA and Luminex assays
- 抗体英文名:
TLE1 biotinylated mouse monoclonal detection antibody, validated for ELISA and Luminex assays
- 靶点:
TLE 1
- 应用范围:
ELISA, LMNX
- 宿主:
Mouse
- 适应物种:
Human, Mouse, Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA700268
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Stored in PBS (pH 7.4) with 0.05% sodium azide, 10mg/ml BSA, 50% glycerol
- 免疫原:
Full length human recombinant protein of human TLE1 (NM_005077) produced in HEK293T cell.
- 规格:
50 ug
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
is an indirect expression of the monoclonal antibody amount in the sample.Methods1 Obtain a 96-well plate and rinse 3x with PBS.2 Add 50 µl of a 1 mg/ml of antigen (venom, serum, or fraction), incubate for 30 min at 37°C.3 Rinse with PBS 3x.4 Add 5% powdered milk
RAT/MOUSE GROWTH HORMONE ELISA KIT
after each wash to remove residual buffer. 8) Add 100 µL Detection Antibody to all wells. Cover the plate with plate sealer and incubate at room temperature for 1 hour on an orbital microtiter plate shaker set to rotate at moderate speed, approximately 400
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