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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mouse Monoclonal UCHL1 / PGP9.5 Antibody
- 抗体英文名:
Mouse Monoclonal UCHL1 / PGP9.5 Antibody
- 靶点:
PGP9.5
- 应用范围:
ICC, IF, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA347068
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide, 50% glycerol, 0.1%BSA
- 免疫原:
The immunogen for UCHL1 antibody: purified recombinant human UCHL1 / PGP9.5 protein fragments expressed in E.coli.
- 规格:
100 ul
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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