Carrier-free (BSA/glycerol-fre
e) MKI67 rat monoclonal antibody, clone OTI5D7 (formerly 5D7)- ￥3999
- OriGene
- CF801577
- 美国
- 2026年04月01日
- FC, WB
- Rat
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Carrier-free (BSA/glycerol-free) MKI67 rat monoclonal antibody, clone OTI5D7 (formerly 5D7)
- 抗体英文名：
Carrier-free (BSA/glycerol-free) MKI67 rat monoclonal antibody, clone OTI5D7 (formerly 5D7)
- 靶点：
Ki67
- 应用范围：
FC, WB
- 宿主：
Rat
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
CF801577
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store at -20°C as received.
- 形态：
Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
- 免疫原：
Human recombinant protein fragment corresponding to amino acids 1160-1493 of human MKI67 (NP_002408) produced in E.coli.
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production
the antigen you want the MAb directed against.If you are raising the MAbs against a small hapten that you coupled to a carrier protein for immunization of the mice,then use the hapten coupled to a different carrier protein for the screen. 4. Expanding
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
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