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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mouse monoclonal anti-NRAS antibody (N-term)
- 抗体英文名:
Mouse monoclonal anti-NRAS antibody (N-term)
- 靶点:
NRAS
- 应用范围:
WB
- 宿主:
Mouse
- 适应物种:
Human, Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA328115
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store at -20°C as received.
- 形态:
PBS with 0.09% (W/V) sodium azide
- 免疫原:
This NRAS antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 70-101 amino acids from the N-terminal region of human NRAS.
- 规格:
400 ul
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
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