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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
DR5 (TNFRSF10B) mouse monoclonal antibody, clone B-K29, Azide Free
- 抗体英文名:
DR5 (TNFRSF10B) mouse monoclonal antibody, clone B-K29, Azide Free
- 靶点:
DR5
- 应用范围:
FN
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM31206AF-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态:
Phosphate-buffered saline without Carrier and preservatives.This product is sterile-filtered through 0.22 µm and treated to remove Endotoxins.State: Azide FreeState: Liquid purified IgG fraction
- 免疫原:
Recombinant Human TRAIL R2/Fc chimera.Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells.
- 规格:
200 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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