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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Goat Polyclonal Antibody against EP4 prostaglandin receptor
- 抗体英文名:
Goat Polyclonal Antibody against EP4 prostaglandin receptor
- 靶点:
EP4
- 应用范围:
WB
- 宿主:
Goat
- 适应物种:
Human (Expected from sequence similarity: Dog)
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA302476
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 克隆性:
Polyclonal
- 保存条件:
Store at -20°C as received.
- 形态:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
- 免疫原:
Peptide with sequence C-LEREVSKNPDLQA, from the internal region of the protein sequence according to NP_000949.1.
- 规格:
100 ug
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文献和实验Staining for Surface Receptors
up" on the coverslip, and the wet whatman paper helps prevent the antibody from drying up. 9. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse
, and apply primary Ab only to the coverslip. Incubate 45'. Dilute antibody with Blotto. Some typical dilutions are listed below: monoclonal M1 mouse anti-Flag antibody (Sigma , Covance ) (Ca+2 sensitive) 1:350-1:500; polyclonal 12CA5 mouse anti-HA (Roche
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
). 9. Secondary antibody to use with goat polyclonal reagent: donkeyanti-goat IgG-horseradish peroxidase (HRP) (sc-2056;Santa Cruz Biotechnology). 10. Secondary antibody to use with rabbit polyclonal reagent: donkey anti-rabbit IgG-HRP (#NA934V, GE
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