- ￥6116
- OriGene
- SM1762P
- 美国
- 2026年04月01日
- FC, IHC
- Rat
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD16 (FCGR3A) rat monoclonal antibody, clone YFC120.5, Purified
- 抗体英文名：
CD16 (FCGR3A) rat monoclonal antibody, clone YFC120.5, Purified
- 靶点：
FCGR3A
- 应用范围：
FC, IHC
- 宿主：
Rat
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
SM1762P
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态：
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative.State: PurifiedState: Liquid purified IgG fraction.
- 免疫原：
Human neutrophils.Spleen cells from Lou rat fused with the rat myeloma cell line. Y3/Ag.1.2.3.
- 规格：
200 ug
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD16
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD16
Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
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