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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Pyroglutamate aminopeptidase rabbit polyclonal antibody, Aff - Purified
- 抗体英文名:
Pyroglutamate aminopeptidase rabbit polyclonal antibody, Aff - Purified
- 应用范围:
ELISA, ID, IF, IP, R, WB
- 宿主:
Rabbit
- 适应物种:
Bovine
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AP21420PU-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 克隆性:
Polyclonal
- 保存条件:
Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.If a slight precipitation occurs upon storage, this should be removed by centrifugation.
- 形态:
PBS (pH 7.2) stabilized with Dextran, without preservatives and foreign proteinsState: Aff - PurifiedState: Lyophilized purified hyperimmune IgG fraction
- 免疫原:
Pyroglutamate aminopeptidase isolated and purified from calf liver.Freund’s complete adjuvant is used in the first step of the immunization procedure.
- 规格:
1 mg
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文献和实验Use of EIA to Measure MMPs and TIMPs
the combination of a monoclonal antibody as the solid-phase with rabbit polyclonal antibody and horseradish peroxidase (HRP)-labeled donkey antirabbit gamma globulin as the second phase (2 ). EIAs for human MMP-9 were developed using MMP-9 specific polyclonal
Tips and hints for the storage of antibodies
! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at ℃. Serum does not contain active proteases
Immunocytology using the HA epitope
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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