- ￥3806
- OriGene
- AM31174AF-N
- 美国
- 2026年04月01日
- FC, FN
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD130 (IL6ST) mouse monoclonal antibody, clone B-K5, Azide Free
- 抗体英文名：
CD130 (IL6ST) mouse monoclonal antibody, clone B-K5, Azide Free
- 靶点：
CD130
- 应用范围：
FC, FN
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM31174AF-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C.
- 形态：
Phosphate-buffered saline without Carrier and preservatives.This prodcut is sterile-filtered through 0.22 µm and treated to remove Endotoxins.State: Azide FreeState: Liquid purified IgG fraction
- 免疫原：
Natural soluble gp130.Hybridoma: Myeloma X63/AG.8653 x Balb/c spleen cells.
- 规格：
200 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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