- ￥5489
- OriGene
- SM293F
- 美国
- 2026年04月01日
- FC
- Mouse
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
CD161 (KLRB1) mouse monoclonal antibody, clone 10/78, FITC
- 抗体英文名：
CD161 (KLRB1) mouse monoclonal antibody, clone 10/78, FITC
- 靶点：
KLRB1
- 应用范围：
FC
- 宿主：
Mouse
- 适应物种：
Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
SM293F
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 克隆性：
Monoclonal
- 保存条件：
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing.
- 形态：
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.Label: FITCState: Liquid purified IgG fraction.Label: Fluorescein Isothiocyanate Isomer 1
- 免疫原：
Purified splenic NK cells from the LEW rat strain.Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
1 - Substrate = ABTS - 100X (from Zymed 00-2001)1 - HRP buffer: 100mM Na Citrate pH 4.2( 490 mg citric acid + 720 mg Na citrate dehydrate + 50 ml H2O, pH 4.2 ). - Hydrogen peroxide 30% ( 1000X ) Protocol: ABSORBTION OF ANTIGEN Dilute Ag to 10 ug/ml in PBS
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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