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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Itgb7 rat monoclonal antibody, clone DATK32, Purified
- 抗体英文名:
Itgb7 rat monoclonal antibody, clone DATK32, Purified
- 靶点:
Itgb7
- 应用范围:
FC, FN, IHC
- 宿主:
Rat
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM08074PU-N
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 形态:
100 mM Borate Buffered Saline, pH 8.2.No preservatives or amine-containing buffer salts added.State: PurifiedState: Liquid purified Ig fraction.
- 免疫原:
AKR/Cum mouse spontaneous T lymphoma cell line TK1. (Ref.1)
- 规格:
500 ug
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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