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- 文献和实验
- 技术资料
- 免疫原:
Rat thymocyte membrane
- 形态:
PBS, without preservativesState: Azide FreeState: Liquid Ig fraction
- 保存条件:
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Rat
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
SM271A
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
FC
- 靶点:
Spn
- 抗体英文名:
CD43 / Leukosialin mouse monoclonal antibody, clone W3/13HLK, Azide Free
- 抗体名:
CD43 / Leukosialin mouse monoclonal antibody, clone W3/13HLK, Azide Free
- 规格:
1 mg
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
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