- ￥3806
- OriGene
- 美国
- AM31219AF-N
- 2026年04月01日
- FC
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Chronic Lymphoïd Leukemia cells (CLL)
- 形态：
Phosphate-buffered saline.Sterile-filtered through 0.22 µm.Carrier and preservative freeState: Azide FreeState: Liquid purified Ig fraction
- 保存条件：
Store the antibody undiluted at 2-8°C.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM31219AF-N
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
FC
- 靶点：
CD7
- 抗体英文名：
CD7 mouse monoclonal antibody, clone B-F12, Azide Free
- 抗体名：
CD7 mouse monoclonal antibody, clone B-F12, Azide Free
- 规格：
200 ug
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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