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- 详细信息
- 文献和实验
- 技术资料
- 形态:
PBS containing 0.09% Sodium Azide as preservative and a stabilizing agent.Label: APC-Cy5.5State: Liquid purified Ig fraction.Label: Allophycocyanin-Cyanine 5.5
- 保存条件:
Store the antibody undiluted at 2-8°C.DO NOT FREEZE!This product is photosensitive and should be protected from light.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM08063AC5-N
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
FC, IF
- 靶点:
Ptprc
- 抗体英文名:
CD45 / LCA (CD45.2 alloantigen) mouse monoclonal antibody, clone 104, APC-Cy5.5
- 抗体名:
CD45 / LCA (CD45.2 alloantigen) mouse monoclonal antibody, clone 104, APC-Cy5.5
- 规格:
100 ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Monoclonal Antibody Production
Order 6 six week old Balb/C mice and let the ARC know they are coming.Have your antigen ready for when they arrive.Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control for monitoring the titer
Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
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