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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse spleen cells.Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line.
- 形态:
PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.Label: PEState: Lyophilized purified IgG fraction.Label: R. Phycoerythrin (RPE)
- 保存条件:
Store the antibody undiluted Prior to and after reconstitution at 2-8°C.DO NOT FREEZE!This product is photosensitive and should be protected from light.
- 克隆性:
Monoclonal
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
SM032R
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Rat
- 应用范围:
FC
- 靶点:
Ptprc
- 抗体英文名:
CD45 / LCA rat monoclonal antibody, clone YW 62.3, PE
- 抗体名:
CD45 / LCA rat monoclonal antibody, clone YW 62.3, PE
- 规格:
100 Tests
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
Cell Surface Immunofluorescence Staining Protocol
. In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD
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