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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
This antibody is derived by fusion of X63-Ag8.653 mouse myeloma cells with spleen lymphocytes from rats repeatedly immunized with glycoconjugates from BALB/c Mouse derived medullary thymic epithelial cells.
- 形态:
PBSState: PurifiedState: Liquid purified IgG fractionPreservative: 0.09% Sodium Azide
- 保存条件:
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM33039PU-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 宿主:
Rat
- 应用范围:
FC, IF, IHC, IP, WB
- 靶点:
Epcam
- 抗体英文名:
Epcam rat monoclonal antibody, clone G8.8, Purified
- 抗体名:
Epcam rat monoclonal antibody, clone G8.8, Purified
- 规格:
100 ug
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文献和实验Cloning of Variable Domains from Mouse Hybridoma by PCR
of thoroughly validated oligonucleotide primers for a PCR procedure to isolate and clone the DNA of antibody Fv fragments (antigen binding region) from mouse and rat hybridoma cell lines. The protocol includes a colony staining assay to identify E. coli clones
问:BAF cell的来源是什么?有什么特性和用途呢?答:ATCC上没有你所谓的BAF cell,DSMZ上有两种BAF cell,如下:Cell line:BA-F8Cell type:mouse hybridoma(anti-rat fiber/myosin)Origin:this hybridoma cell line was described to secrete a mouse monoclonal antibody(IgG2b)that reacts with rat type
Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
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