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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Human IL-2
- 形态:
PBSState: Azide FreeState: Lyophilized purified Ig
- 保存条件:
Prior to reconstitution store at 2-8°C.Following reconstitution store the antibody undiluted at 2-8°C for one monthor (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性:
Monoclonal
- 适应物种:
Human
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM00961AF-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
ELISA, FN
- 靶点:
IL2
- 抗体英文名:
IL2 mouse monoclonal antibody, clone B025M, Azide Free
- 抗体名:
IL2 mouse monoclonal antibody, clone B025M, Azide Free
- 规格:
0.2 mg
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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