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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
-
- 抗体英文名:
,Oct-4 (3F5); Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7.
- 靶点:
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- 浓度:
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- 应用范围:
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- 宿主:
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- 适应物种:
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- 保质期:
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- 抗原来源:
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- 目录编号:
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- 级别:
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- 库存:
99
- 供应商:
爱必信(上海)生物科技有限公司
- 标记物:
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- 克隆性:
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- 保存条件:
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
- 形态:
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- 亚型:
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- 免疫原:
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- 规格:
100ul
| 概述 | |
| 别名 | Oct-4 (3F5); Oct3; Oct4; Otf3; Otf4; NF-A3; Oct-3; Oct-4; Otf-3; Otf-4; Otf3g; Oct3/4; Oct-3/4; Otf3-rs7. |
| 宿主 | Mouse |
| 反应种属 | Mouse |
| 应用 | WB: 1/500-1/1000 |
| 分子量 | Detects a band of approximately 45 kD(Predicted molecular weight: 38 kD) Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| 免疫原 | Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli. |
| 性能 | |
| 形式 | Monoclonal Antibody |
| 纯化方法 | Affinity Purified |
| 克隆号 | 3F5 |
| 同种型 | IgG2b |
| 储存/保存方法 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 存储溶液 | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| 修饰 | Unmodified |
| 靶标 | |
| 背景说明 | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| UniProt | P20263 |
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文献和实验Phenotyping of Mouse Models with OCT
Optical coherence tomography (OCT) is an invaluable technique to perform noninvasive retinal imaging in small animal models such as mice. It provides virtual cross sections that correlate well with histomorphometric data with the advantage
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Activity of the Germline-Specific Oct4-GFP Transgene in Normal and Clone Mouse Embryos
recombination). Transgenes have been generated using different regions of the Oct4 gene promoter to drive expression of either lacZ or GFP, which allows the activity of the Oct4 promoter to be followed in living cells and tissues. The bacterial LacZ gene encodes
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