- ￥4980.18
- nsjbio
- RQ4401
- 2026年03月31日
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A human partial recombinant protein corresponding to amino acids N960-F1245 was used as the immunogen for the NEDD4 antibody.
- 亚型：
Rabbit IgG
- 适应物种：
Human
- 宿主：
Rabbit
- 规格：
100 ug
Primary antibody
纯化方法：
Antigen affinity purified
通用信息：
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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文献和实验Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
step 5 min Antibody 15 min PBS Wash 6 x 2 min Protein A Gold 15 min PBS Wash 6 x 2 min Water Wash 5 x 1 min. Blocking Any "sticky" protein can be used to cover non-specific binding sites on the thawed sections
Protein A Purification of Antibody
1. Reagents(1) Affi-gel Protein-A Agarose (BioRad #153-6153)(2) MAPS II Binding Buffer (BioRad # 153-6161)(3) 0.314 g/ml diH2O(4) MAPS II Elution Buffer (BioRad #153-6162)(5) 0.023 g/ml diH2O(6) MAPS II Regeneration Buffer (BioRad #153-6166(7) 1M
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