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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 适应物种:
Human, Rabbit, Monkey
- 库存:
100
- 宿主:
Rabbit
- 应用范围:
IHC-P
- 靶点:
Human Prostaglandin I2 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- 规格:
50 μg
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文献和实验) 2、Input 条带清晰,co-IP 条带很弱? 设X为诱饵蛋白,Y 为靶蛋白,有可能为 X 和 Y 的相互作用本身比较弱 X 蛋白并不是 Y 的唯一,只部分 Y 与 X 互作 可以尝试反向拉,比如用 Y 去拉 X,可能效果比较好 3、我的 p 53 蛋白正好和抗体重链分子量接近,要如何减少抗体干扰? 建议方案: IP/WB 一抗使用不同种属来源,选择与IP抗体种属无交叉反应的二抗(Cross Adsorbed secondary antibody) 特殊二抗:比如仅识别重链或者轻链的二抗,或者仅
A in two separate Eppies and equilibrate 3x with 500µl EBC-buffer (i.e. add EBC buffer, mix gently, spin down and remove supernatant) a. 1x for antibody binding: add 20µl RFP + 400µl EBC-buffer to equilibrated beads and incubate for 1h
Aspects of CNS Lupus: Mouse Models of Anti-NMDA Receptor Antibody Mediated Reactivity
of interest binds the N -methyl-d -aspartate receptor (NMDAR), a receptor for glutamate that is a major excitatory neurotransmitter in the brain involved in synaptic plasticity, in memory and learning, and in emotional responses. We introduce basic
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