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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
液体形式,冰袋运输
- 英文名:
Bovine Factor V/ Va
- 供应商:
安诺伦(北京)生物科技有限公司
- 规格:
1.0 mg
研究领域:Cofactors
产品概述:Purified from freshly collected bovine plasma using a combination of salt precipitations and column chromatography. This protein is a mixture of Factor V/Va and intermediate activation products as judged by 4-20% gradient gels. Bovine Factor Va functions as a cofactor with the serine protease Factor Xa in the activation of prothrombin. This protein may be used as a source of V/Va in the Prothrombinase complex.
存储溶液:Buffer composition = 20 mM HEPES-HCl / 0.15 M NaCl / 50% glycerol / pH 7.4 Extinction Coefficient (1%) = 9.6/17.4 One unit = 10 μg Molecular Weight = 330,000; 94,000; 74,000; 71,000; 31,000 daltons Concentration determined by BCA
简单描述:BFV/Va, Purified from freshly collected bovine plasma using a combination of salt precipitations and column chromatography. This protein is a mixture of Factor V/Va and intermediate activation products as judged by 4-20% gradient gels. Bovine Factor Va functions as a cofactor with the serine protease Factor Xa in the activation of prothrombin. This protein may be used as a source of V/Va in the Prothrombinase complex.
详情:Related Products Bovine Factor Xa
生物商城:http://www.annoron.com
办公电话:010-56256916
官方售后:400-965-8633
企业 QQ:3212874516、3198592576
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文献和实验: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Research) or a Perkin Elmer Cetus cycler was used. The amplification products were separated by gel electrophoresis (3 V/cm) through a 1.6% gel (0.8% agarose and 0.8% Synergel "(Diversified BioTech, Newton Centre, CT))(9) in recirculating TAE " buffer.
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