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PFKL Antibody (Center K433)

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  • $495
  • Leading Biology
  • APR06073G
  • 2026年03月31日
  • WB, E
  • Rabbit
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      371-399 aa

    • 亚型

      Rabbit Ig

    • 保存条件

      Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

    • 适应物种

      Human

    • 库存

      100

    • 宿主

      Rabbit

    • 应用范围

      WB, E

    • 靶点

      This PFKL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 371-399 amino acids from the Central region of human PFKL.

    • 规格

      100 μl

    Prouduct: We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality PFKL Antibody (Center K433). Functiong: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Summary: Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.

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    图标文献和实验
    相关实验
    • Generation of Antibody Molecules Through Antibody Engineering

      Twenty-five years ago, Georges K�hler and C�sar Milstein invented a means of cloning individual antibodies, thus opening up the way for tremendous advances in the fields of cell biology and clinical diagnostics ( 1 ). However, in spite

    • Double immunohistochemistry for BCL6 and PNA for Germinal Center detection.

      (see Reagents and Solutions). 3- incubate with the blocking for 10 min. If one of your antibody is biotin-conjugated, you need at this point to do endogenous biotin blocking. 4- blot the slides without washing and apply the primary antibody, in a moist

    • Antibody Purification

      to 1 liter with Qcheck to make sure pH is 3.0(2) Affigel Blue Running Buffer10 mM K2HPO4 2.28 g K2HPO40.15 M NaCl 8.2 g NaCl0.02% azide 0.2 g Na azideup to 1 liter with Q(3) 1.4 M NaCl8.1 g NaClup to 100 ml with Q(4) Saturated NH4SO4767 g NH4SO4add 1 liter Q

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