- $495
- Leading Biology
- United States
- APR09157G
- 2026年05月01日
- Rabbit
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
Rabbit Ig
- 保存条件：
Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.
- 库存：
100
- 宿主：
Rabbit
- 靶点：
This Phospho-Caspase 9-S196 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S196 of human caspase 9.
- 抗体名：
MCH6
- 规格：
100 μl
46281 Da
细胞定位：
形式：
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
参考文献：
Martin, M.C., et al., J. Biol. Chem. 280(15):15449-15455 (2005). Raina, D., et al., J. Biol. Chem. 280(12):11147-11151 (2005). Cornelis, S., et al., Oncogene 24(9):1552-1562 (2005). Mohammad, R.M., et al., Mol. Cancer Ther. 4(1):13-21 (2005). Tacconi, S., et al., Exp. Neurol. 190(1):254-262 (2004).
产品概述：
We constantly strive to ensure we provide our customers with the best antibodies. As a result of this work we offer this antibody in purified format. We are in the process of updating our datasheets. If you have any questions regarding this update, please feel free to contact our technical support team. This product is a high quality Phospho-Caspase 9(S196) antibody.
功能：
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf- 1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP- ribose) polymerase (PARP).
总结：
Caspase 9 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
联系方式：
生物商城：http://www.annoron.com
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官方售后：400-965-8633
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文献和实验或 C6 cells 作为 cleaved caspase-3 的阳性参照。更多靶点的阳性对照，请登陆CST网站——研究——靶标的阳性对照处理。图二 蛋白质印迹分析（图源自CST Caspase-3 Antibody #9662 抗体说明书）为什么所有的实验都要设置对照？无论是初高中刚接触生物学实验设计还是上研究生后发表 SCI 文章，老师们以及 reviewer 都会敲着「黑板」强调强调再强调，每一个实验都必须有对照！因为设置适当的阴性、阳性甚至空白对照对于实验结果的准确、有效阐释至关重要！阅历丰富
【求助】请问western blot做凋亡检测时，识别caspase-3酶原和激活的caspase-3的抗体是相同的吗？
-caspase-3（#9664）和原形态的caspase-3（#9662），但是需要说明的是，原形态的caspase-3抗体也可以同时检测剪切态的caspase-3，所以建议购买原形态的caspase-3就行了。 （如下图所示） 另外，PARP也是购买全长的就行了，（PARP Antibody #9542）。 lsdcfhyj 谢谢两位的回答 本文由丁香园论坛提供，想了解更多有用的、有意思的前沿资讯以及酷炫的实验
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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