M TLR4 Antibody (N-term) [APR1

分子量：95519

细胞定位：Cell membrane; Single-pass type I membrane protein. Early endosome {ECO:0000250|UniProtKB:O00206}. Cell projection, ruffle. Note=Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis Colocalizes with RFTN1 at cell membrane and then together with RFTN1 moves to endosomes, upon lipopolysaccharide stimulation {ECO:0000250|UniProtKB:O00206}

功能：Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:9851930, PubMed:9989976, PubMed:20133493). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:24380872). Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (By similarity). Activated by the signaling pathway regulator NMI which acts as damage- associated molecular patterns (DAMPs) in response to cell injury or pathogen invasion, therefore promoting nuclear factor NF-kappa-B activation (By similarity).

总结：Tissue Location: Expressed in macrophages (at protein level) (PubMed:28098138). Highly expressed in heart, spleen, lung and muscle Lower levels are found in liver and kidney (PubMed:23812099)

稀释方法：WB--1:1000