- ￥3275.10
- Merck millipore
- 05-379
- 2026年03月30日
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文献和实验per mouse, using a 20G needle, intraperitonealy. Collect ascites when animals are ready (swollen abdomen), 10 days to 15 days after injection. Spin fluid at 3000 g for 10 min. to remove the cells. If there is an oil layer, remove it and discard
Monoclonal Antibody Production
Positive Clones (1) The day prior to any expansion,obtain feeder cells.For this first expansion from the 200 µl cultures in 96-well plates to 500 µl cultures in 24-well plates,add 200 µl of feeder cells/well in DMEM/20% FCSHT to the 24-well plates the day
monoclonal anti-HA 16B12 (MMS101R, BAbCo, used at 1:200 to 1:1000 dilution) mouse monoclonal anti-HA 12CA5 (12CA5, Boehringer, used at 1:200 dilution) rabbit polyclonal anti-HA (RS1010C, BAbCo, used at 1:200 dilution) Cy3-conjugated affinity
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