- ￥3186
- Abcam
- ab215969
- 2026年03月30日
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- 克隆性：
多克隆
- 规格：
100ug
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文献和实验Phenotype-specific immunodetection of cyclins using 488/630 nm dual laser flow cytometry
antibody against surface marker of interest 0.1% paraformaldehyde in PBS 70% EtOH in glycerol (stored at 4°C) staining buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 m g/ml solution with 100 U/ml DNase-free
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
staining buffer (1% BSA in PBS with 0.005% Tween 20 and 0.1% sodium azide) propidium iodide (50 µ g/ml solution with 100 U/ml DNase-free RNase)
Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
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