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文献和实验Replication timing by density transfer
Calbiochem's Pronase (catalog #53702), which seems pretty clean--i.e., plates spread after Pronase treatment aren't contaminated--but I heard from someone who'd used Sigma's Pronase and had massive growth of Streptomyces. 20x SCP = 0.6 M Na2 HPO
【信息】Northstar BioProducts（NSBP）
)2 asparagine-1, 4-N-acetyl--glucosaminohydrolase. Lyophilized powder Activity: ≥25 units/mg protein 100465-1 Endoglycosidase H, (Streptomyces griseus), EC 3.2.1.96 0.1 unit 100465-2 Endoglycosidase H, (Streptomyces griseus), EC 3.2.1.96
In Situ HYBRIDIZATION TO TISSUE SECTIONS
2O. Pronase treat: -Remove the slides from the rack and place horizontally in a tray. -With a Pasteur pipet, carefully drip ‰0.25mg/ml pronase solution onto the sections and incubate 10' (thhe incubation time starts with treatment
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