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- 库存：
大量
- 供应商：
西宝生物
- 规格：
500ML
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
the plants the day before infiltation). Draw vacuum for 15-20 min for WS and 30 min for Col-0 at a pressure close to 0.05 Bar (we are using an oil pump). 7. Before removing the plants from the vacuum jar place a plastic bag over the pot and beaker. Pull
-crack to help liberate the DNA. Check that the solution actually freezes. Overlay with a drop of mineral oil and incubate at 60°C for 60 minutes, followed by 95°C for 15 minutes. Cool to 4°C. Pipette 22.5 l of PCR master mix
QuikChange (Stratagene)This is a quick and reliable (and dead easy too!) method for PCR but costs more. It is, however, highly recommended if you need to make only a small number of mutants, or if you can't or won't do any molecular biology
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