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文献和实验Identification and Characterization of Small GTPase-Associated Kinases
-activated kinase (PAK) (2 ,3 ); the p160 RhoA-associated kinase (ROK) (4 ,5 ); and the neutrophil Wiscott-Aldrich Syndrome protein (WASP) (6 ). Deletion analysis of a number of cDNAs encoding these proteins have shown the p2l-binding domains encompass
Bisulfite sequencing of small DNA/cell samples
well when starting with large amounts of DNA. Here we describe a protocol for small numbers of cells and little DNA which requires some specific handling. The protocol is based on a strategy originally introduced by our lab (Hajkova et al. , 2002) using
Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
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