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文献和实验Bicinchoninic Acid (BCA) Protein Assay (Smith)
to 562 nm.Glass or polystyrene (cheap)cuvettes may be used. Procedure 1 (standard assay) Reagents 1.Reagent A: 1 gm sodium bicinchoninate (BCA),2 gm sodium carbonate,0.16 gm sodium tartrate,0.4 gm NaOH,and 0.95 gm sodium bicarbonate,brought to 100 ml
galactosidase assay for cells transfected with pSV- -Galactosidase Vector
uL. Add 150uL of 2X assay buffer to each tube. Vortex briefly. Incubate at 37oC for 30 min or until yellow color has developed. Stop the reaction by adding 500uL of 1M sodium Carbonate. Your total volume is now 1mL. Vortex Read
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 3C-Ligated Immunoprecipitated Fragments 46. Using standard cloning protocols, clone the 3C-ChIP products (from Step 44) into a vector that has enzyme overhangs similar to those generated in the 3C assay. This step typically
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