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文献和实验YPD FILLED MICROTITER PLATES Uses: 1. Grow individual strains to use for PCR 2. Shipping strains 1. Make YPD regular agar (1.5-2%) for sending strains. 2. Make 200ml batches = enough for 6 microtiter
Preparing Antibiotics Stock Solution and Ampicillin Agar Plates
, which sometimes has pH=9-10 adjusting the pH can be important! Preferred storage temperature is -70°C! See also here: Effects of Storage Temperature and pH on the Stability of Eleven P-Lactam Antibiotics in MIC Trays. JOURNAL OF CLINICAL MICROBIOLOGY, Mar. 1985, p
for strains grown in SCD; 10 ul should be used for strains grown in YPD. 6. Invert a few times, and pour onto a pre-warmed plate containing the appropriate solid media. Swirl to cover the plate evenly. This step should be done quickly to prevent the agar
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