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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50G
- 供应商:
西宝生物
- 保存条件:
室温
- 英文名:
DEXTRAN MW200,000,000 PYROGEN FREE
- 规格:
800 NMOL
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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
Protein Syntheses in Cell Free Systems
Materials •Suspension culture of fibroblast cells (1 liter)•35 mM Tris-HCl,pH 7.4,140 mM NaCl (TBS buffer)•10 mM Tris-HCl,pH 7.5,10 mM KCl,and 1.5 mM magnesium acetate (TBS-M)•10X TBS-M200 mM Tris-HCl,pH 7.5,1200 mM KCl,50 mM magnesium acetateand 70
Purification of Inhibitor-Free Soil DNA Obtained by other Methods
实验步骤 1. Dissolve the DNA pellet with 200 ul of Elution Buffer or Buffer TE. Important: DNA pellet should be dissolved in Elution Buffer or Buffer TE(pH8.0). 2. Add 50 ul inhibitor Removal Resin to the sample
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