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文献和实验Northern Blot I. Electrophoresis clean gel box with NaOH and/or SDS, 2 hours to overnight, rinse with water prepare Agarose gel solution [1 % Agarose, 1 x MOPS, H 2 O to 95 % of endvolume ]
The Northern Blot Hybridization
c) Hyb conditions too stringent. d) Washing conditions too stringent. Hybridization of Ribosomal RNA: a) Too much total RNA has been blotted. Do not exceed 20ug of total RNA
The Neverfail Northern Blot Hybridizatio
. d) Washing conditions too stringent. Hybridization of Ribosomal RNA: a) Too much total RNA has been blotted. Do not exceed 20ug of total RNA or try to use poly A+ instead. b) Probe is too sticky. Increase the stringency of the hyb conditions
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