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文献和实验Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
Inc.) per well. Invert gently several times during a 5 minute room temperature incubation. Let stand at room temperature for 1 minute before transferring to filter plate. 7. Using a wide bore pipette tip, transfer the lysate to a Qiagen TurboFilter™ (Qiagen
for 2 minutes. 2) Drop 20 μL of peptoid sheet solution on a plasma-treated silicon substrate. Allow to sit for 3 minutes. Remove excess solution with tip of Kim-wipe. Pipette 20 μL of water onto the surface and remove excess solution
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
buffer R1 with RNAse A (0.2 mg/ml) and RNAse T1 (100 units/ml). Let rest for 10 min. on ice to relax pellets before gentle vortexing or shaking. [1] 4. Add 300 ul of Qiagen buffer R2 (lysis buffer) per well. Seal wells with plastic sealing tape and mix
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