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文献和实验Making Boiled Donkey (or whatever) Serum (BDS) for Blocking
Mounting Media 20mM Tris pH 8.0 0.5% N-propyl gallate 90% Glycerol Store at 4oC PHEM (500 mls) 2x 18.14 g Pipes 6.5 g Hepes 3.8 g EGTA 0.99 g MgSO4 pH 7.0 w/ KOH PBS (5x in 500 mls) 20.45 g NaCl
James Hardwick CNBr Cleavage Procedure
in deionized H2O after the transfer is complete. This removes any residual Tris-glycine that seems to affect the migration of the CNBr fragments during SDS-PAGE. 2. Cut out the piece of NC containing the protein and immediately put it into a microfuge
RUVKUN Antibody Staining Protocol revised 10/29/90
1% BSA 1X PBS 0.5% Triton X-100 0.05% Na Azide 1mM EDTA TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES: 50 mM Tris Cl pH 9.5 5 mM MgCl 2% agarose -dissolve 2 mg n-propyl gallate in 70
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