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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
, xylene, eukitt, Paraffin wax , anti-DNA-POD (A2), Phosphate Buffered Saline (PBS), 3,3'-diaminobenzidine tetrahydrochloride (DAB) tablets (A2), Phosphate-citrate buffer , Mayer's hematoxylin. A.2.2. Methodology A) Immunocytochemical
Immunohistochemisty-Enzymatic Protocol
# P6148) and 14 (v/v) saturated picric acid (Sigma Catalog # 925-40), pH 6.9. Picric acid is optional. Sucrose Solution: 130 mM Na2HPO4, 30 mM KH2PO4, 10 (w/v) sucrose (Sigma Catalog # S7903), 0.01 sodium azide (Sigma Catalog # S2002) and 0.03
Bromodeoxyuridine Immunohistochemistry
, add 1 drop of B, vortex, and let sit 30 min. RT. 15. 1X PBS, 3 min.X 5, RT. 16. ABC, 30 min., RT. 17. 1X PBS, 3 min. X 3. 18. pH 7.5 buffer, 15 min., RT: 1 drop pH 7.5 buffer in 2.5 ml house distilled water Do not use ddH2O and PBS
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