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文献和实验Use of the B.D. FACS or Calibur Flow Cytometers
should be repeated for the remaining photomultiplier tubes: FL1: FITC, GFP, YFP, Hoechst FL2: Phycoerythrein (PE), Propidium Iodide (DNA content) FL3: TriColor, Propidium Iodide (viability) FL4: DAPI (LSR machine) Note if you are using P.I. for a DNA
Single Primer ("Semi-Random") P
nuclease @ 1 u/µL 1 hr x 37° Purify using Qiaquick (or related) technology, elute in 30 µL TE Check 3 µL on 1% agarose gel Sequence with primer Pn 2. Long Method Stringency optimization is done with Po, and reamplification using
Agarose Gel Electrophoresis for the Separation of DNA Fragments
bacteriophages and other viruses by agarose-gel electrophoresis. J. Virol. 14, 1235-1244 (1974). 4. Smith, S.B., Aldridge, P.K., & Callis, J.B. Observation of individual DNA molecules undergoing gel electrophoresis. Science. 243, 203-206 (1989).
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