Anti-Her-4.1/4.2 (IN-2), Z-Fis

Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discs

for detection of the FISH signals BSA (Sigma, 98% electrophoresis purity) Normal donkey or goat serum (Jackson laboratories) Blocking solution: PBS 1X; 0.3% Triton X-100; 1% BSA; 10% normal donkey or goat serum FITC-anti-Biotin

Detection of apoptotic process in situ using immunocytochemical and TUNEL assays

, xylene, eukitt, Paraffin wax , anti-DNA-POD (A2), Phosphate Buffered Saline (PBS), 3,3'-diaminobenzidine tetrahydrochloride (DAB) tablets (A2), Phosphate-citrate buffer , Mayer's hematoxylin.   A.2.2. Methodology  A) Immunocytochemical

Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes

of the FISH signal Add 20µl of the diluted FITC-anti-Biotin antibody (1/500e in PBS + 4% BSA) on the slide. Cover with a coverslip and incubate for 1 hour at RT in a humid chamber in the dark. From now on, perform all incubations in the dark;