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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
ABCB1 / P Glycoprotein Mouse anti-Human Monoclonal (JSB-1) Antibody
- 宿主:
详见产品说明书
- 抗原来源:
详见产品说明书
- 供应商:
上海西宝
- 是否单克隆:
0
- 规格:
100 µl
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Protocol for anti-HA antibody Western Blotting
seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes again(in 1XPBS
Protocol for anti-HA antibody Western Blotting
it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes
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