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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Lyophilized or Liquid
- 保存条件:
-20°C
- 克隆性:
Polyclonal
- 宿主:
Rabbit
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 抗体名:
Cy7标记的心脏特异性丝氨酸蛋白酶抗体
- 规格:
100ul
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文献和实验Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
Fluorochrome Absorption and Emission Spectra
. The efficiency of the light energy transfer between the two fluorochromes can be seen in Fig. 2F where less than 5% of the absorbed light is lost as fluorescence at 575 nm by R-PE. Compared to other fluorescence energy-transfer systems used in flow cytometry (e.g
Fluorochrome Absorption and Emission
. The efficiency of the light energy transfer between the two fluorochromes can be seen in Fig. 2F where less than 5% of the absorbed light is lost as fluorescence at 575 nm by R-PE. Compared to other fluorescence energy-transfer systems used in flow cytometry (e.g
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