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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant Vaccinia virus Cell surface-binding protein (1-304AA)
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
-20°C or -80°C
- 克隆性:
多克隆
- 标记物:
Non-conjugated
- 适应物种:
Vaccinia virus
- 抗原来源:
12个月
- 目录编号:
Vaccinia virus
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB
- 靶点:
D8L
- 抗体英文名:
D8L Antibody
- 抗体名:
D8L Antibody
- 规格:
100μg/50μg
| 规格: | 100μg | 产品价格: | ¥1320.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥880.0 |
|
Product Name |
D8L Antibody |
|
Code |
CSB-PA322653LA01VAA |
|
Aliases |
D8LCell surface-binding protein antibody; Carbonic anhydrase homolog antibody |
|
Product Type |
Polyclonal Antibody |
|
Immunogen Species |
Vaccinia virus |
|
Size |
100μg/50μg |
|
Uniprot ID |
P20508 |
|
Immunogen |
Recombinant Vaccinia virus Cell surface-binding protein (1-304AA) |
|
Host Species |
Rabbit |
|
Species Reactivity |
Vaccinia virus |
|
Tested Applications |
ELISA, WB |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Conjugate |
Non-conjugated |
|
Buffer |
Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
|
Form |
Liquid |
|
Stroage |
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|
Purification Method |
>95%, Protein G purified |
|
Target Names |
D8L |
|
Research Areas |
Others |

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文献和实验Analysis of Protein Complexes by 2D Blue Native/SDSPAGE and Antibody-Shift Assay
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful strategy for the separation of native multiprotein complex (MPC). Combined with other techniques, such as SDS–PAGE, immunoblotting, mass spectrometry, and antibody-shift
has eluted into the column. c) Add 5mL of PBS on top. d)Open Stopcock. collect in 2mL fractions, discarding the first 2mL and keeping the 2nd 2mL fraction. The second 2mL fraction contains the conjugated antibody*. *NOTE: Step d) appropriate only if 1mL
(either undiluted or 1:1 with PBT) or diluted polyclonal antiserum and incubate at 4 C overnight 8. Rinse with PBT 3 times, then wash 4 times for 15 minutes each 9. Add secondary antibody (usually 1:200 in PBT) and incubate 2 hours at room temperature
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