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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Peptide sequence around phosphorylation site of serine 217/221 (I-D-S(p)-M-A) derived from Human MEK1/MEK2.
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
-20°C or -80°C
- 克隆性:
多克隆
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse, Rat
- 抗原来源:
12个月
- 目录编号:
Homo sapiens (Human)
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB, IHC
- 靶点:
MAP2K1
- 抗体英文名:
Phospho-MAP2K1 (Ser217/Ser221) Antibody
- 抗体名:
Phospho-MAP2K1 (Ser217/Ser221) Antibody
- 规格:
100μl
|
Product Name |
Phospho-MAP2K1 (Ser217/Ser221) Antibody |
|
Code |
CSB-PA297521 |
|
Aliases |
Dual specificity mitogen activated protein kinase kinase 1 antibody; Dual specificity mitogen-activated protein kinase kinase 1 antibody; ERK activator kinase 1 antibody; MAP kinase kinase 1 antibody; MAP kinase/Erk kinase 1 antibody; MAP2K1 antibody; MAPK/ERK kinase 1 antibody; MAPKK 1 antibody; MAPKK1 antibody; MEK 1 antibody; Mek1 antibody; MEKK1 antibody; Mitogen activated protein kinase kinase 1 antibody; MKK 1 antibody; MKK1 antibody; MP2K1_HUMAN antibody; PRKMK1 antibody; Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody; Protein kinase mitogen activated, kinase 1 antibody; protein kinase mitogen-activated kinase 1 antibody |
|
Product Type |
Polyclonal Antibody |
|
Immunogen Species |
Homo sapiens (Human) |
|
Size |
100μl |
|
Uniprot ID |
Q02750 |
|
Immunogen |
Peptide sequence around phosphorylation site of serine 217/221 (I-D-S(p)-M-A) derived from Human MEK1/MEK2. |
|
Host Species |
Rabbit |
|
Species Reactivity |
Human, Mouse, Rat |
|
Tested Applications |
ELISA, WB, IHC; WB:1:500-1:1000 |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Conjugate |
Non-conjugated |
|
Buffer |
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
Form |
Liquid |
|
Stroage |
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|
Purification Method |
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
|
Target Names |
MAP2K1 |
|
Research Areas |
Signal transduction |

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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
neutralization of phospho-specific antibodies with phospho-peptides immobilized on magnetic beads. This technique allows for sequestration of antibody–peptide complex from the incubation solution, minimizing the risk of formation of unblocked antibodies capable
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