Phospho-CFL1 (Tyr139) Polyclo
nal Antibody（多克隆抗体） | Phospho-CFL1 (Tyr139) Antibody | Phospho-CFL1 (Tyr139)抗体- ￥2454
- CUSABIO
- 武汉
- CSB-PA266541
- 2026年03月28日
- ELISA, IHC
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C or -80°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human, Mouse, Rat
- 抗原来源：
12个月
- 目录编号：
Homo sapiens (Human)
- 供应商：
武汉研升生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, IHC
- 靶点：
CFL1
- 抗体英文名：
Phospho-CFL1 (Tyr139) Antibody
- 抗体名：
Phospho-CFL1 (Tyr139) Antibody
- 规格：
100μl
|
Product Name |
Phospho-CFL1 (Tyr139) Antibody |
|
Code |
CSB-PA266541 |
|
Aliases |
18 kDa phosphoprotein antibody; CFL 1 antibody; CFL antibody; CFL1 antibody; COF1_HUMAN antibody; Cofilin 1 antibody; Cofilin 1 non muscle antibody; Cofilin antibody; Cofilin non muscle isoform antibody; Cofilin-1 antibody; epididymis secretory protein Li 15 antibody; HEL-S-15 antibody; non-muscle isoform antibody; p18 antibody |
|
Product Type |
Polyclonal Antibody |
|
Immunogen Species |
Homo sapiens (Human) |
|
Size |
100μl |
|
Uniprot ID |
P23528 |
|
Immunogen |
Peptide sequence around phosphorylation site of tyrosine 139 (N-C-Y(p)-E-E) derived from Human cofilin. |
|
Host Species |
Rabbit |
|
Species Reactivity |
Human, Mouse, Rat |
|
Tested Applications |
ELISA, IHC; IHC:1:50-1:100 |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Conjugate |
Non-conjugated |
|
Buffer |
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
Form |
Liquid |
|
Stroage |
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|
Purification Method |
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
|
Target Names |
CFL1 |
|
Research Areas |
Neuroscience；Cancer；Cardiovascular;Signal transduction |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 antibody (A) and Total-p38 antibody (B). Cell Based Elisa were performed directly in the 96-well plates using Phospho-p38 and Total-p38 antibodies (C). Figure 3: JNK detection with FACECells were serum-starved for 16 hours
signaling公司的Phospho-PI3K p85 (Tyr458)/p55(Tyr199) 我自己分析没有条带无非是细胞状态不好，或者抗体不好，不知道还有没有可能是没有加磷酸化酶抑制剂，以至于磷酸化的蛋白被去磷酸化了？ 谢谢啦～ yhwangcams 检测内参蛋白如GAPDH,ACTIN,TUBULIN是否成功？ 用PLB裂解细胞得到的蛋白适合做REPORTER，做WESTERN不太合适。建议可以用其他BUFFER裂解蛋白。
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